Name the  enzyme used in PCR.

  a)DNA Polymerase           b)Cellulase      c)Taq polymerase        d)Chitinase.

  Observe the sequence given below. 

  5'---- GAA TT C------3

     3'---- CT T AAG------5'

            a)  Identify the above DNA sequence and give its  significance.

            b) Name the enzyme used to cut this DNA sequence.

Name the enzyme used to cut the DNA at specific point

            a)DNA polymerase                 b)Restriction endonulcease                

            c) DNA ligase                        d) Protease

 Name the two core techniques that enabled the birth of modern biotechnology.

Restriction endonuclease is   used to cut  DNA at specific sequences?

     a) Write the name of first discovered restriction endonuclease?

      b) Which are the basic rules of naming  restriction enzymes?

 Identify the  techniques used for the following.

    a) Separation of DNA fragments

    b) Amplification of DNA

     c) Large scale purification and preservation of products from bioreactor

Choose the odd one and give reason.

            Hind III, EcoR I, BamH I,amp^R

Observe the figure given below.

a) Identify  the  selectable markers present in this vector.

b) What is the use of these selectable markers?   

A gene of interest can be amplified in vitro into millions of copies in a  limited time period.The diagram given below shows its basic steps.

a ) Name this technique.

b) Identify Step I,Step II,Step III

 c) Name the DNA polymerase used in this technique .

Match the items in column A and B.