Name the organism from which the enzyme Taq polymerase used in PCR isolated

Agrobacterium tumifaciens is a pathogen of several dicot plants. Name the plasmid present in this bacterium.

Stanley Cohen and Herbert Boyer constructed an artificial recombinant DNA by linking an antibiotic resistant gene into the plasmid of a bacteria.

a) Name this bacteria?.

b) Write the three basic steps in genetic modification of an organism.

 Observe the sequence given below.

5’----GAATTC-------3’

3’----CTTAAG-------5’

1.  Suggest a name for this type of sequence.Define it.

2. What is the significance of this sequence?

pBR 322 is a commonly used cloning vector in genetic engineering.

a) What does ori in pBR 322 stand for?.

b) Write the significance of ori and rop sequences.

Expand Ti plasmid.

Name the bacterium from which this plasmid is isolated. Write its significance.

Expand PCR.

Write the name and peculiarity of DNA polymerase enzyme used in PCR..

In order to cut DNA with restriction enzymes ,DNA has to be isolated from the host cell and to be purified.

a) Name two enzymes used to break open cells during isolation of DNA.

b) Explain how DNA is purified during this process?

c) Name the process of removal of DNA  after purification.

What do you mean by recombinant protein?. Give example.

Disarmed pathogens can be effectively used to deliver genes of our interest into bacterial , plant and animal cells.

a.Give two examples for such pathogens used in biotechnology.

b.Give an account of micro-injection.

Ti plasmid is used for making transgenic plants. It  is obtained from.

            a)E.coli  b)Salmonella typhimurium   c) Agrobacterium  tumifaciens       d)Bacillus thuringiensis

Write the significance of the bacterium Thermus aquaticus  in  r DNA technology?

Name the  enzyme used in PCR.

  a)DNA Polymerase           b)Cellulase      c)Taq polymerase        d)Chitinase.

  Observe the sequence given below. 

  5'---- GAA TT C------3

     3'---- CT T AAG------5'

            a)  Identify the above DNA sequence and give its  significance.

            b) Name the enzyme used to cut this DNA sequence.

Name the enzyme used to cut the DNA at specific point

            a)DNA polymerase                 b)Restriction endonulcease                

            c) DNA ligase                        d) Protease

 Name the two core techniques that enabled the birth of modern biotechnology.

Restriction endonuclease is   used to cut  DNA at specific sequences?

     a) Write the name of first discovered restriction endonuclease?

      b) Which are the basic rules of naming  restriction enzymes?

 Identify the  techniques used for the following.

    a) Separation of DNA fragments

    b) Amplification of DNA

     c) Large scale purification and preservation of products from bioreactor

Choose the odd one and give reason.

            Hind III, EcoR I, BamH I,amp^R

Observe the figure given below.

a) Identify  the  selectable markers present in this vector.

b) What is the use of these selectable markers?   

A gene of interest can be amplified in vitro into millions of copies in a  limited time period.The diagram given below shows its basic steps.

a ) Name this technique.

b) Identify Step I,Step II,Step III

 c) Name the DNA polymerase used in this technique .

Match the items in column A and B.