Write the applications of DNA fingerprinting.

Various steps of DNA fingerprinting is given below arrange them in proper order.

(i) isolation of DNA,

(ii) separation of DNA fragments by electrophoresis,

(iii) detection of hybridised DNA fragments by autoradiography.

(iv) digestion of DNA by restriction endonucleases,

(v) hybridisation using labelled VNTR probe,

(vi) transferring (blotting) of separated DNA fragments to synthetic

Observe the diagram and give the answers

(a) Name the process .

(b)  a, b and c are the steps of this process, identify them.

Mention the main three linkages  present in a nucleotide of DNA?

Describe the termination process of transcription in bacteria.

The percentage of nucleotide A in DNA isolated from human liver is observed to be 30.7%, What is the expected percentage of a)T, b)G, c)C

Note the relation between the first two and find the missed  one.

i)Operon concept: Jacob and Monad : :Central dogma:_______

ii)Polymerase I: rRNA :: RNA Polymerase II:_____________

iii)Termination codon: UAG ::  Intiation codon :______________

Genetic information is copying from one strand of DNA to RNA by an important process

 (a) Name the process mentioned here?

(b) Which are the steps involved in this process?

Complete the Table