There are three critical research areas of biotechnology.Which are they ?

The first clinical gene therapy was given to a  4 year old girl.
a) What is gene therapy?.
b) What was her genetic disorder?

Define biopiracy.

GM crops have been useful in many ways.  Write any four merits of GM crops.

Animals that possess extra gene are called transgenic animals.

a)Name the first transgenic cow.

b)List out any four beneficial aspects of the transgenic organisms

Gene therapy helps to correct certain genetic diseases.

a) Name a hereditary disease that can be corrected by gene therapy.

b) Mention other two treatment methods used to cure the above identified disorder.

Choose the correct answer.

Crystals of Bt toxin produced by some bacteria do not kill that bacteria because:

a) Bacteria encloses toxin in a special sac.

b) Toxin is soluble.

c) Bacteria can resist toxin.

d)  Toxin is inactive.

How is ADA deficiency treated by Gene Therapy?

Can a disease be detected before its symptoms appear? What are the methods used for this?

Choose the correct answer.

Maturation of proinsulin into insulin takes place after

            a)Joining of C peptide             b)Removal of disulphide bridge

            c)Removal of B chain           d) Removal of C peptide

Name and explain the novel strategy adopted to prevent nematode infection in tobacco plants?

Some hereditary diseases can be corrected by gene therapy.

a) What do you mean by gene therapy?

b) Expand ADA.

 Rosie is a transgenic cow. How milk produced by  Rosie differ from that of other cows?

In 1983 an American company Eli Lilly produced human insulin artificially.

a) Write  the method of production of artificial insulin.

b) Write the effect in humans of using insulin from animal sources.

Explain how Bt toxin works in the body of boll worms?

Genetic engineering helps to overcome  the limitation of traditional hybridization procedure in plant and animal breeding.

  a) What is the limitation of  traditional hybridization procedure mentioned here?

 b) Explain how genetic engineering helps us to overcome this limitation?

Match the items in column A with column B  .

           A                                               B

a) Bt Cotton                                 1. ADA deficiency

b) RNAi                                         2.Eli Lilly

c) Insulin                                      3.Insecticidal protein

d) Gene therapy                          4.Silencing of mRNA

Bt cotton is a transgenic plant which contain the genes of Bacillus thuringiensis that code for  toxic proteins..

a)Name two genes which code for these toxic proteins.?

b)Name  the toxic protein produced by these genes?

Meloidegyne incognitia is a nematode parasite infects the roots of tobacco plants. Its infection can be prevented by a novel strategy.

a) Name this strategy.

b) Explain the principle behind this strategy

Observe the figure.

a) Identify A,B and C.

b) Name the bacteria  which is used for the production of genetically engineered insulin?

How do Bt cotton differ from normal cotton?

Hereditary diseases can be corrected  by gene therapy.

a) Name a hereditary disease that can be corrected by gene therapy.

b) Mention other two remedial measures to cure this disorder.

Expand GEAC. Write the role of GEAC.

ELISA based on the principle of ______________________.

Gene therapy is a collection of methods that allows correction of a gene defect that has been diagonised in a child/ embryo.

a.How correction of a gene defect can be done?

b.Name the disease for which first clinical gene therapy was given.

Fill in the blank.

 Human protein obtained from transgenic animal which is used to treat emphysema is  ......................................

Choose the correct answer.

The bacterium used as vector for DNA transfer to induce RNAi in tobacco plant is:

A. E.coli

B. Agrobacterium tumifaciens

C. Bacillus thuringiensis

D. Salmonella typhimurium

Transgenic animals are developed to produce biological products to treat certain human diseases. Name such a biological product and it's use.

Choose the correct answer.

The bacterium used as vector for DNA transfer to induce RNAi in tobacco plant is:

A. E.coli

B. Agrobacterium tumifaciens

C. Bacillus thuringiensis

D, Salmonella typhimurium

Bt cotton is an example of GMO. Expand Bt.